We use cookies to provide visitors of our website with the best possible experience. Identical data time-windows were then selected for both the initial and final nm and nm fluorescence intensity readings, and the average of the data points within each data window was determined to obtain: Figure 10 demonstrates the dose response of increasing concentrations of barium chloride in regard to inhibiting depolarization. The Bio-Tek Synergy HT has a 4-Zone temperature control system that ensures superior temperature uniformity necessary for kinetic assays. In addition- the ability to use filters with different bandpasses allows the investigator to tailor their fluorescence measurements to their experiment more effectively. In the HTS and Drug Discovery environments, microplate-based assays are developed to make determinations on large numbers of samples.

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More products from this supplier. Using well-mode kinetics, fluorescence intensity measurements were obtained from cells in the polarized state using both sets of filter-pairs prior to the addition of depolarizing stimulant.

The absorbance optics are a single-channel system that has the capability of measuring absorbance from the UV to the near IR range.

Instrument Description and Capabilities: The energy transfer takes place without the generation of a photon, with the rate of energy transfer being dependent on, among other things, the degree of overlap in spectrums and the proximity of the donor and acceptor.

Luminescent assays are sensitive and quantitative tools commonly used for a variety of purposes in biomedical and pharmaceutical research. Synergy HT utilizes two independent sets of optics to provide uncompromised performance. Todays research requires that instrumentation be cost effective- reliable and easy to use.


Synergy™ HT: A Multi-Mode Microplate Reader for Today’s High Performance Luminescence Research

Pharmaceutical and biotechnology research requires instrumentation to be both functional and versatile. An upgrade to CFR 21 Part 11 compliant software is also available. A confirmation has been sent to you, please click the link to verify your email address and activate your subscription.

The data presented using the calcium indicator Fluo-4 demonstrate that the Synergy HT with Injector can be used for calcium flux assays. Fluorescence determinations are made using a tungsten halogen lamp with interference filters in conjunction with a PMT detector for maximum sensitivity.

Syjergy results are indicated by shaded areas.

The Synergy HT collected 80 data points, once every 0. Figure 6 shows typical data obtained from the Synergy HT.

Bio-Tek Synergy HT Multi-Detection Microplate Reader

Each syringe is connected to syhergy separate injector tip that can be located adjacent to the top detector probe or above the bottom probe. Fluo 4 Assay Development To assess the versatility of the Synergy HT, preliminary studies were performed to see if Fluo 4 Molecular Probes F calcium assays could be quickly developed.

Pipetting robot Precision BioTek Instruments. The monochromator provides wavelength selection from to nm in 1 nm increments. The use of a xenon flash lamp allows for both Ibotek and visible light absorbance measurements.

BioTek Synergy HT

It is the distance dependence that is utilized with VSPs. Each syringe is connected to a separate injector tip. Luminescence measurements are performed using the fluorescence optics path with the excitation lamp turned off and an excitation filter replaced with an opaque plug to ensure a dark reading chamber.

Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorine. It is represented by the following formula: Besides saving time and reagents, the ability to assess all drug concentrations at one time eliminates the experiment-to-experiment variation associated with multiple experiments.


Absorbance microplate reader Epoch BioTek Instruments.

Synergy HTX Multi-Mode Microplate Reader: Fluorescence-Luminescence – Overview

Specially designed fluorescent interference filters prevent light leakage from excitation wavelength to the emission wavelength- particularly important with fluorescent compounds with small Stokes shifts.

The FRET-based detection method provides ratiometric results, which significantly reduces errors arising from well-to-well variations in cell number, dye loading and signal intensities, plate inconsistencies, and temperature fluctuations. A xenon-flash lamp is used to illuminate a high-precision, diffraction-grating monochromator.

For absorbance measurements, there is a xenon-flash lamp with a monochromator for wavelength selection and biitek detection. As demonstrated in Figure 12, while the fluorescence in cells that received only HBSS increased, cells that received Ionomycin returned a much more rapid and greater increase than the HBSS-only wells.

Ascom invests further into its strategic partners business by appointing When making fluorescence determinations- the Bio-Tek Synergy HT uses a tungsten halogen lamp with interference filters for wavelength specificity in conjunction with a PMT detector. Medicus announces diagnostic-data mobility initiative Medicusa Vienna-based digital health company, is proud to announce Ionomycin induces the release of calcium from the endoplasmic reticulum ER that can be detected by Fluo 4 present in the loaded cells.